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ABSTRACT Celery (Apium graveolens L., Apiaceae) is one of the popular aromatic vegetables and part of the daily diet around the world. In this study, aqueous-ethanolic and hexane extracts of celery seed were prepared and the amount of n-butylphthalide, as an active component, was determined in each extract. Then the effects of hexanic extract on systolic, diastolic, mean arterial blood pressure and heart rate were evaluated in an invasive rat model. The vasodilatory effect and possible mechanisms of above mentioned extracts on aorta ring were also measured. High performance liquid chromatography analysis revealed that hexanic extract contains significantly higher amounts of n-butylphthalide, compared to aqueous-ethanolic extract. The results indicated that hexanic extract significantly decreased the systolic, diastolic, mean arterial blood pressure and heart rate in normotensive and hypertensive rats. Our data revealed that celery seed extract exerts its hypotensive effects through its bradycardic and vasodilatory properties. Moreover, the active components in celery seed extracts could induce their vasodilatory properties through Ca2+ channel blocking activity in endothelial and non-endothelial pathways and particularly by interference with the extra or intracellular calcium.
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Objective: To develop a quantitative analysis of multi-components with single marker (QAMS) and validate its accuracy and feasibility for simultaneously determining 3-n-butylphthalide (b), sedenenolide (sen), and sedanolide (san) in celery seed extracts (CSE). Methods: Three main effective components,3-n-butylphthalide, sedenenolide, and sedanolide, were selected as analytes for evaluating the quality of CSE. The relative correction factors (RCF, f) of 3-n-butylthalide to the other two thalides were calculated: fb/sen = 0. 226, fb/san = 0.702. The external standard method was used to determine the title compounds in 6 batches of CSE and the results were compared with those of QAMS. Results: The RSD of RCF calculated by different instruments was between 4.4%-6.7%. The RSD of RCF calculated by the same instrument with different chromatographic columns was between 2. 3%-3. 6%. The established RCF had a good reproducibility. The quantitative results of three thalides determined by external standard method and QAMS method were not significantly different. Conclusion: The present method can serve as a new mode to determine multi-components in CSE when standard substances are unavailable.
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Objective: The present study was carried out to explore protective effects of ethanolic extract of Apium graveolens (celery seeds) on ritonavir, a protease inhibitor induced dyslipidemia. Materials & Methods: Thirty mice were divided into 5 groups. Group 1 mice served as healthy control. Group 2 mice were given drug ritonavir at doses of 33.33 mg/kg (200mg/day, human dose), group 3 received same dose of ritonavir and ethanolic extract of celery seeds(CSE) at the doses of 75mg/kg. Group 4 was treated with same dose of ritonavir and CSE at high doses i.e.150mg/kg. Group 5 mice were given ritonavir and hypolipidemic drug, fenofibrate. All groups of mice were given the drug and extract by oral gavage route for the period of 12 weeks. Blood lipid profile and liver lipids of all the groups were tested at the end of 12 weeks. Results: Blood lipid profile was found to be deranged in the group of mice treated with ritonavir. Concurrent treatment of ritonavir with low dose of CSE showed no significant improvement in blood lipid profile in group 3 mice but high dose CSE along with ritonavir with the same dose of ritonavir exhibited significant improvement (p<0.05) in group 4 mice. Effect of fenofibrate in group 5 was almost equally effective as that of high dose of CSE. There was a similar pattern of decrease in liver lipids in all the groups (p<0.05). Conclusion: Above results suggest that ethanolic extract of celery seeds possess potential for improving blood lipid profile & liver lipids deranged by ritonavir when given concurrently. Its efficacy approaches that of fenofibrate. Its intake along with ritonavir would be better in terms of cost and side effects as compared to fenofibrate.
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OBJECTIVE:To observe the antiaging effect of celery seed oil (CSO) on aging mice and rats induced by D-galactose. METHODS: Aging models were established with D-galactose hypo. At the same time, the model animals were intragastrically given CSO. After 6 weeks, the effects of CSO on learning and memory were evaluated by step down test and electrophysiological test. And the MDA content, MAO-B and SOD activity in brains of aging mice were measured. RESULTS: CSO can markedly improve the learning and memory of aging mice and rats, reduce the content of MDA and activity of MAO-B, and increase SOD activity. CONCLUSION: CSO has good antiaging effect.